Document Type : Original Article
Authors
- Shahrzad Sadat Eftekharvaghefi 1, 2
- Mohammad Akbarpour 2
- Fatemeh Shahsavari 3, 4
- Mohammad Ehsan Khani 5
- Amir Mahdi Kouhsefidi Gouderi 5
- Maryam Iranpour 2
- Reza Bahramabadi 2
- Sara Rashidi Nejad 2
- Reza Malek pour Afshar 2
1 Department of Medicine, Ke.C., Islamic Azad University, Kerman, Iran
2 Pathology and Stem Cell Research Center, Kerman University of Medical Sciences, Kerman, Iran
3 Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
4 Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran
5 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
Abstract
Background: Antibiotic resistance is a worldwide concern that decreases the beneficial effects of antibiotics. Escherichia coli is a prevalent etiological agent of infections, particularly within hospital intensive care units. Therefore, comprehending the antibiotic resistance profile of E. coli is essential for effective treatment. This study evaluated the antibiotic resistance characteristics of E. coli collected from patients admitted to Afzalipour hospital in Kerman, Iran.
Methods: This retrospective study examined 139 patients hospitalized at Afzalipour hospital between September 2017 and August 2019. E. coli isolates from clinical specimens were evaluated for antibiotic susceptibility using disk diffusion and agar dilution techniques. The data were analyzed with a chi-square test in SPSS.
Results: Cefazolin and cefotaxime were the most resistant drugs within the examined population. In contrast, gentamicin and amikacin demonstrated reduced resistance rates. The resistance level varied based on the sample type and the department where the patients were admitted.
Conclusion: Excessive and wrong uses of antibiotics develop antibiotic resistance in E. coli. Therefore, gentamicin and amikacin can be considered to reduce antibiotic resistance challenges.
Keywords
- Antibiotic resistance
- Antibacterial drug resistance
- Urinary tract infections
- Escherichia coli infection
Main Subjects
- Caine LA, Nwodo UU, Okoh AI, Ndip RN, Green E. Occurrence of virulence genes associated with diarrheagenic Escherichia coli isolated from raw cow’s milk from two commercial dairy farms in the Eastern Cape province, South Africa. Int J Environ Res Public Health. 2014;11(11):11950- 63. doi: 10.3390/ijerph111111950.
- Tille PM. Bailey and Scott’s Diagnostic Microbiology. 13th ed. St. Loius: C.V. Mosby Company; 2013.
- Mandal SM, Roy A, Ghosh AK, Hazra TK, Basak A, Franco OL. Challenges and future prospects of antibiotic therapy: from peptides to phages utilization. Front Pharmacol. 2014;5:105. doi: 10.3389/fphar.2014.00105.
- Pavlopoulou A. RecA: a universal drug target in pathogenic bacteria. Front Biosci (Landmark Ed). 2018;23(1):36-42. doi: 10.2741/4580.
- Kollef MH, Fraser VJ. Antibiotic resistance in the intensive care unit. Ann Intern Med. 2001;134(4):298-314. doi: 10.7326/0003-4819-134-4-200102200-00014.
- Basavaraju M, Gunashree BS. Escherichia coli: an overview of main characteristics. In: Starčič Erjavec M, ed. Escherichia coli-Old and New Insights. IntechOpen; 2022. doi: 10.5772/ intechopen.105508.
- Wassenaar TM. Insights from 100 years of research with probiotic coli. Eur J Microbiol Immunol (Bp). 2016;6(3):147- 61. doi: 10.1556/1886.2016.00029.
- Liu Y, Zhu M, Fu X, Cai J, Chen S, Lin Y, et al. Escherichia coli causing neonatal meningitis during 2001-2020: a study in eastern China. Int J Gen Med. 2021;14:3007-16. doi: 10.2147/ijgm.S317299.
- Govindarajan DK, Eskeziyaw BM, Kandaswamy K, Mengistu DY. Diagnosis of extraintestinal pathogenic Escherichia coli pathogenesis in urinary tract infection. Curr Res Microb Sci. 2024;7:100296. doi: 10.1016/j.crmicr.2024.100296.
- Ozgurhan G, Vermezoglu O, Ocal Topcu D, Karbuz A, Vehapoglu A, Hacihamdioglu B. Escherichia coli meningitis after rotavirus gastroenteritis in an infant. Case Rep Infect Dis. 2016;2016:1909260. doi: 10.1155/2016/1909260.
- Croxall G, Hale J, Weston V, Manning G, Cheetham P, Achtman M, et al. Molecular epidemiology of extraintestinal pathogenic Escherichia coli isolates from a regional cohort of elderly patients highlights the prevalence of ST131 strains with increased antimicrobial resistance in both community and hospital care settings. J Antimicrob Chemother. 2011;66(11):2501-8. doi: 10.1093/jac/dkr349.
- Abushaheen MA, Muzaheed, Fatani AJ, Alosaimi M, Mansy W, George M, et al. Antimicrobial resistance, mechanisms and its clinical significance. Dis Mon. 2020;66(6):100971. doi: 10.1016/j.disamonth.2020.100971.
- Cui X, Lü Y, Yue C. Development and research progress of anti-drug resistant bacteria drugs. Infect Drug Resist. 2021;14:5575-93. doi: 10.2147/idr.S338987.
- Nasrollahian S, Graham JP, Halaji M. A review of the mechanisms that confer antibiotic resistance in pathotypes of E. coli. Front Cell Infect Microbiol. 2024;14:1387497. doi: 10.3389/fcimb.2024.1387497.
- Eltai NO, Al Thani AA, Al Hadidi SH, Al Ansari K, Yassine HM. Antibiotic resistance and virulence patterns of pathogenic Escherichia coli strains associated with acute gastroenteritis among children in Qatar. BMC Microbiol. 2020;20(1):54. doi: 10.1186/s12866-020-01732-8.
- Jalal Mardane AM, Rahele Darfashi, Amin Shayeshitapour. Examining the antibiotic resistance profile of E. coli bacteria strains isolated from infants and children admitted to 22 Bahman Gonabad Hospital in 2015. The 12th Annual Research Congress of Eastern Medical Sciences Students Country; 2016.
- Mohammadzadeh R, Mohammadi-Gollou A, Salehabadi Y. The prevalence of CTX-M beta-lactamase gene in urinary tract infections in patients referring to Bonab city hospitals. Iran J Med Microbiol. 2019;13(4):302-9. doi: 10.30699/ ijmm.13.4.302.
- Mollazaeh A, Gholami MS, Shahi A, Najafipour S, Mobasheri F, Ashraf Mansuri J, et al. Evaluation of antibiotic resistance pattern of isolated gram-negative bacteria from urine culture of hospitalized patients in different wards of Vali-Asr hospital in Fasa during the Years 2012 and 2013. J Adv Biomed Sci. 2014;4(3):275-83. [Persian].
- Heidari-Soureshjani E, Heidari M, Doosti A. Epidemiology of urinary tract infection and antibiotic resistance pattern of E. coli in patients referred to Imam Ali hospital in Farokhshahr, Chaharmahal va Bakhtiari, Iran. J Shahrekord Univ Med Sci. 2013;15(2):9-15. [Persian].
- Hamed Mulla Abbaszadeh KE, Hamidi M, Asdalahi M. Antibiotic sensitivity and resistance pattern in Escherichia coli strains isolated from clinical samples of Arad hospital, Tehran in 2017-2018. Iran J Infect Dis Trop Med. 2013;18(60):59-63. [Persian].
- Hajigholami Esfahani Z, Zargar M, Yari R, Rezaei N. Investigating the antibiotic resistance pattern of Escherichia coli strains isolated from urinary tract infections in Qom city. Appl Biol. 2012;3(12):23-9. [Persian].
- Razeghi Z, Goli HR, Salehian M, Gholami M. Evaluation of antimicrobial resistance pattern in Escherichia coli strains isolated from patients referred to teaching hospitals of Mazandaran University of Medical Sciences. Alborz Univ Med J. 2023;12(1):35-41. doi: 10.61186/aums.12.1.35. [Persian].
- Roshani M, Goudarzi H, Hashemi A, Ardebili A, Erfanimanesh S, Bahramian A. Detection of IS903, IS26 and ISEcp1 elements in CTX-M-producing Klebsiella pneumoniae and Escherichia coli isolates from patients with leukemia in Iran. Jundishapur J Microbiol. 2018;11(12):e58929. doi: 10.5812/jjm.58929.
- Hassanen FS, Sabike II, Haikel GI, Elged EA. Screening for antimicrobial resistance in some pathogens isolated from cold smoked fish marketed in Menofiya government. Benha Vet Med J. 2018;35(2):269-83. doi: 10.21608/bvmj.2018.96302.
- Linggarjati S, Parti DD, Sakinah EN. Antibiotic sensitivity on pathogenic bacteria causing bacterial vaginosis. J Obstet Gynecol Sci. 2021;29(1):18-22. doi: 10.20473/mog. V29I12021.18-22.
- Xiao L, Totten AH, Crabb DM, Atkinson TP, Waites KB. Antimicrobial susceptibilities and mechanisms of resistance of commensal and invasive Mycoplasma salivarium isolates. Front Microbiol. 2022;13:914464. doi: 10.3389/ fmicb.2022.914464.
- Ma L, Siu LK, Lin JC, Wu TL, Fung CP, Wang JT, et al. Updated molecular epidemiology of carbapenem-non-susceptible Escherichia coli in Taiwan: first identification of KPC-2 or NDM-1-producing E. coli in Taiwan. BMC Infect Dis. 2013;13:599. doi: 10.1186/1471-2334-13-599.
- Lindemann PC, Risberg K, Wiker HG, Mylvaganam H. Aminoglycoside resistance in clinical Escherichia coli and Klebsiella pneumoniae isolates from Western Norway. APMIS. 2012;120(6):495-502. doi: 10.1111/j.1600- 0463.2011.02856.x.
- Stiawan LO, Kamal A, Mudasir M, Suherman S. Synthesis of amikacin modified carbon dots-doped nitrogen and zinc for Escherichia coli detection. Key Eng Mater. 2023;949:143-51. doi: 10.4028/p-kA7lzU.
- Herdiyanti H, Alimsardjono L, Indiastuti DN. Resistance patterns of Escherichia coli and Klebsiella pneumoniae bacteria against amikacin, ceftazidime, meropenem, nitrofurantoin antibiotics in elderly patients with UTI in RSUD Dr. Soetomo General Hospital Surabaya. JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga. 2019;10(1):20-4. doi: 10.20473/juxta.V10I12019.20-24.
- Seni J, Falgenhauer L, Simeo N, Mirambo MM, Imirzalioglu C, Matee M, et al. Multiple ESBL-producing Escherichia coli sequence types carrying quinolone and aminoglycoside resistance genes circulating in companion and domestic farm animals in Mwanza, Tanzania, harbor commonly occurring plasmids. Front Microbiol. 2016;7:142. doi: 10.3389/ fmicb.2016.00142.
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