Journal of Kerman University of Medical Sciences

Cytotoxic Properties of Methanolic Extracts from Quercus Infectoria and Terminalia Chebula on A549 and PC12 Cancer Cell Lines: A Comparative Study Based on Tannin-rich and Tannin-free content

Document Type : Original Article

Authors

Saam Noroozi 1
Ahmad Gholamhosseinian 2
Beydolah Shahouzehi 3
Seyed Noureddin Nematollahi-Mahani 4
Fariba Sharififar 5
Mahsa Maleknia 1
Seyedeh Hamideh Jalaeian Haddad 6

1 Department of Biochemistry, Fasa University of Medical Sciences, Fasa, Iran

2 Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran

3 Gastroenterology and Hepatology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran

4 Kerman Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

5 Department of Pharmacognosy, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran

6 Environmental Health Engineering Department, Faculty of Health, Mashhad University of Medical Sciences, Mashhad, Iran

https://doi.org/10.34172/jkmu.4162
Abstract
Introduction: Quercus infectoria and Terminalia chebula are traditional medicinal plants in the Middle East, known for their significant therapeutic properties. This study aimed to investigate the cytotoxic effects of tannin-rich and tannin-free methanolic extracts, derived from these plants, on A549 (human lung adenocarcinoma) and PC12 (mouse pheochromocytoma) cell lines.
Methods: The extracts were prepared from air-dried plant tissues using a maceration technique. Cytotoxicity was assessed using four different concentrations (1, 10, 50, and 100 μg/mL) using Trypan Blue and WST-1 assays. The half-maximal inhibitory concentrations (IC50) were determined for both cell lines.
Results: The IC50 values revealed that Quercus infectoria exhibited stronger cytotoxic activity compared to T. Chebula. In A549 cells, Quercus infectoria showed IC50 values of 80 μg/mL (tannin-rich) and 102 μg/mL (tannin-free), whereas Terminalia chebula demonstrated 104 μg/mL (tannin-rich) and 111 μg/mL (tannin-free). For PC12 cells, the IC50 values for Quercus infectoria were 68 μg/mL (tannin-rich) and 103 μg/mL (tannin-free), while Terminalia chebula recorded 80 μg/mL (tannin-rich) and 129 μg/mL (tannin-free).
Conclusion: This study confirms that tannin-rich extracts exhibited greater cytotoxic effects than their tannin-free counterparts, emphasizing the role of tannins in influencing anticancer activity. The findings suggest that tannin-mediated toxicity is a key factor in the observed differences, providing valuable insights into the mechanistic contributions of tannins to cancer cell suppression, which warrants further research into their bioactive mechanisms.

Keywords

Quercus infectoria
Terminalia chebula
A549
PC12
Cytotoxicity
Subjects
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Journal of Kerman University of Medical Sciences
Volume 33
Pages 1-6

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