Journal of Kerman University of Medical Sciences

Current Issue

By Issue

By Author

Author Index

Keyword Index

About Journal

Aims and Scope

JKMU Ethical Policies

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

News

Metric

Correlation of Aging-Related Anxiety with Dentate Gyrus and Protective Effect of Rosemary Extract

Document Type : Original Article

Authors

1 Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran

2 Tissue Engineering Department, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran

3 Department of Anatomy, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran

4 Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Aging is an unavoidable process that causes destructive changes to the brain. However, using plants with unique properties, such as rosemary, may help prevent complications related to aging. Therefore, we aimed to evaluate the effect of rosemary on anxiety associated with aging.
Methods: Male Wistar rats were divided into two groups: young and old, each with two subgroups: old rats receiving solvent (SO), old rats receiving rosemary extract (EO), young rats receiving solvent (SY), and young rats receiving rosemary extract (EY). After 12 weeks, anxiety behavior was tested through the elevated plus maze. Real-time RT-PCR was used to evaluate BDNF expression in the dentate gyrus region. Finally, the thickness of the granular layer and intact neurons in the dentate gyrus were assessed by Nissl staining.
Results: The results showed a significant reduction in the percentage of entries and number of entrances into the open arms in aged rats compared to young groups (p ≥ 0.05). However, the administration of rosemary significantly improved anxiety compared to the SO group (p ≥ 0.05). Additionally, administration of rosemary (100 mg/kg) proved a significant elevation of BDNF expression in the treated rats compared to the SO group (p ≥ 0.05). Also, aging meaningly decreased the thickness of the granular layer and the number of intact neurons in the dentate gyrus compared to other groups (p ≤ 0.05).
Conclusion: Normal aging can increase anxiety and tissue changes in the dentate gyrus, while it seems that rosemary extract improves anxiety and can reduce neuronal damage in the dentate gyrus.

Highlights

Mohsen Eslami Farsani (Google Scholar) (PubMed)

Keywords

Main Subjects

References
  1. Petersen PE, Kandelman D, Arpin S, Ogawa H. Global oral health of older people-call for public health action. Community dent health. 2010;27(4):257-67. doi: 10.1922/CDH_2711Petersen11.

 

  1. Ince Yenilmez M. Economic and social consequences of population aging the dilemmas and opportunities in the twenty-first century. Appl Res Qual Life. 2015;10:735-52. doi: 10.1007/s11482-014-9334-2.

 

  1. Gaspar-Silva F, Trigo D, Magalhaes J. Ageing in the brain: mechanisms and rejuvenating strategies. Cellular and Molecular Life Sciences. 2023;80(7):190. doi: 10.1007/s00018-023-04832-6.

 

  1. Xiao QX, Liu Q, Deng R, Gao ZW, Zhang Y. Postoperative cognitive dysfunction in elderly patients undergoing hip arthroplasty. Psychogeriatrics. 2020;20(4):501-9. doi: 10.18502/jost.v9i3.13035 .

 

  1. Hung C-W, Chen Y-C, Hsieh W-L, Chiou S-H, Kao C-L. Ageing and neurodegenerative diseases. Ageing res rev. 2010;9:S36-S46. doi: 10.1016/j.arr.2010.08.006.

 

  1. Weeden CS, Roberts JM, Kamm AM, Kesner RP. The role of the ventral dentate gyrus in anxiety-based behaviors. Neurobiol Lear Mem. 2015;118:143-9. doi: 10.1016/j.nlm.2014.12.002.

 

  1. Ghasemi M, Navidhamidi M, Rezaei F, Azizikia A, Mehranfard N. Anxiety and hippocampal neuronal activity: Relationship and potential mechanisms. Cognitive, Affective, & Behavioral Neurosci. 2022;22(3):431-49. doi: 10.3758/s13415-021-00973-y.

 

  1. Salazar-Colocho P, Lanciego JL, Del Rio J, Frechilla D. Ischemia induces cell proliferation and neurogenesis in the gerbil hippocampus in response to neuronal death. Neurosci res. 2008;61(1):27-37. doi: 10.1016/j.neures.2008.01.008.

 

  1. González-Minero FJ, Bravo-Díaz L, Ayala-Gómez A. Rosmarinus officinalis L.(Rosemary): An ancient plant with uses in personal healthcare and cosmetics. Cosmetics. 2020;7(4):77. doi: 10.3390/cosmetics7040077.

 

  1. Rahbardar MG, Hosseinzadeh H. Therapeutic effects of rosemary (Rosmarinus officinalis L.) and its active constituents on nervous system disorders. Iranian j basic med sci.2020;23(9):1100. doi: 10.22038/ijbms.2020.45269.10541.

 

  1. Saroya AS, Singh J, Saroya AS, Singh J. Neuropharmacology of Lavender, Rosemary and Salvia. Pharmacot Potent Natural Products Neurol Dis. 2018:43-58. doi: 10.1007/978-981-13-0289-3_4.

 

  1. Pengelly A, Snow J, Mills SY, Scholey A, Wesnes K, Butler LR. Short-term study on the effects of rosemary on cognitive function in an elderly population. J med food. 2012;15(1):10-7. doi: 10.1089/jmf.2011.0005.

 

  1. Abbasi S, Khaledi N, Askari H. High intensity interval training increases the expression of hippocampus BDNF gene and decreases the serum tnf-α in Diabetic Rat. Med. J. Tabriz Univ. Med. Sci. 2020;42(5):591-600. doi: 10.34172/mj.2020.083.

 

  1. Bakhshayesh A, Farsani RE, Seyedebrahimi R, Ababzadeh S, Heidari F, Farsani ME. Evaluation of the Negative Effects of Opium Tincture on Memory and Hippocampal Neurons in the Presence of Chicory Extract. Adv Biomed Res. 2023;12(1):23. doi: 10.4103/abr.abr_210_21.

 

  1. Amarya S, Singh K, Sabharwal M. Ageing process and physiological changes. Gerontol. 2018;32:137-44. doi: 10.5772/intechopen.76249.

 

  1. Segal DL, Qualls SH, Smyer MA. Aging and mental health: John Wiley & Sons; 2018. doi: 10.1002/9781119133186.

 

  1. Habtemariam S. The therapeutic potential of rosemary (Rosmarinus officinalis) diterpenes for Alzheimer’s disease. J Evid Based Complementary Altern Med. 2016;2016. doi: 10.1155/2016/2680409.

 

  1. Soori R, Vahdat H, Shabkhiz F, Ababzadeh S, Eslami Farsani M. Effects of Aerobic Exercise and Rosemary Extracts on Inflammatory Factors in Cerebellar of Male Old Rats. Qom Univ. Med. Sci. J. 2020;14(4):11-21. doi: 10.29252/qums.14.4.11.

 

  1. Boiangiu RS, Brinza I, Hancianu M, Erdogan Orhan I, Eren G, Gündüz E, et al. Cognitive facilitation and antioxidant effects of an essential oil mix on scopolamine-induced amnesia in rats: Molecular modeling of in vitro and in vivo approaches. Molecules. 2020;25(7):15-19. doi:10.3390/molecules25071519.

 

  1. Aghamiri SM, Farsani ME, Seyedebrahimi R, Sarikhani MJ, Ababzadeh S. Synergic Effects of Rosemary Extract and Aerobic Exercise on Sperm Parameters and Testicular Tissue in an Aged Rat Model. GCT. 2022. doi: 0.5812/gct-130832.

 

  1. Rasoolijazi H, Mehdizadeh M, Soleimani M, Nikbakhte F, Farsani ME, Ababzadeh S. The effect of rosemary extract on spatial memory, learning and antioxidant enzymes activities in the hippocampus of middle-aged rats. Med J Islam Repub Iran. 2015;29:187. PMID: 26034740.

 

  1. Rasoolijazi H, Ofogh SN, Ababzadeh S, Mehdizadeh M, Shabkhiz F. Comparing the Effects of Rosemary Extract and Treadmill Exercise on the Hippocampal Function and Antioxidant Capacity in Old Rats. Basic Clin Neurosci. 2021;12(3):361. doi: 10.32598/bcn.12.3.2139.1.

 

  1. Khalid A, Abbasi UA, Amber S, Mirza FJ, Asif M, Javed A, et al. Methylphenidate and Rosmarinus officinalis improves cognition and regulates inflammation and synaptic gene expression in AlCl 3-induced neurotoxicity mouse model. Mol Biol Rep. 2020;47:7861-70. doi: 10.1007/s11033-020-05864-y.

 

  1. Ferreira FF, Ribeiro FF, Rodrigues RS, Sebastião AM, Xapelli S. Brain-derived neurotrophic factor (BDNF) role in cannabinoid-mediated neurogenesis. Frontiers in cellular neurosci. 2018;12:441. doi: 10.3389/fncel.2018.00441.

 

  1. Bathina S, Das UN. Brain-derived neurotrophic factor and its clinical implications. Arch Med Sci. 2015;11(6):1164-78. doi: 10.5114/aoms.2015.56342.

 

  1. Achour M, Ben Salem I, Ferdousi F, Nouira M, Ben Fredj M, Mtiraoui A, et al. Rosemary tea consumption alters peripheral anxiety and depression biomarkers: a pilot study in limited healthy volunteers. JANA. 2022;41(3):240-9. doi: 10.1080/07315724.2021.1873871.

 

  1. Shahraki FK, Nabiuni M, Farhadi M, Amini E. The Effect of Hydroalcoholic Rosmarinus Officinalis (Rosemary) on Memory Retention Deficit in Young Offspring Rat Induced by Maternal Sleep Deprivation. Pharm Biomed Res. 2020. doi: 10.18502/pbr.v6i2.3808.

 

  1. Atef RM, Fattah IOA, Mahmoud OM, Abdel-Rahman GM, Salem NA. Protective effects of Rosemary extract and/or Fluoxetine on Monosodium Glutamate-induced hippocampal neurotoxicity in rats. Rom J Morphol Embryol. 2021;62(1):169. doi: 10.47162/RJME.62.1.16.
Journal of Kerman University of Medical Sciences
Volume 32
Pages 1-6
Files
Share
How to cite
Statistics