Journal of Kerman University of Medical Sciences

Annexin A5 sensitizes HCT 116 cells to the cytotoxic effects of cisplatin

Articles in Press

Document Type : Original Article

Authors

Puria Rostampour 1
Jamileh Saberzadeh 1
Bahareh Zamani 1
Soofia Sorourian 2
Mohammad Ali Takhshid 1, 2

1 Division of Medical Biotechnology, Department of Laboratory Sciences, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

2 Diagnostic Laboratory Sciences and Technology Research Centre, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

Abstract
Background: Chemoresistance is a significant obstacle that hinders the effectiveness of cisplatin. This study aimed to explore the potential effect of annexin A5 (ANXA5) on sensitizing HCT116 cells to the cytotoxic effects of cisplatin.
Methods: A recombinant pCMV6-ANXA5-IRES-GFP plasmid was utilized to overexpress ANXA5 in HCT116 cells. The plasmids were transfected using Lipofectamine 3000, and the ANXA5 expression was quantified using quantitative real-time PCR (q-PCR). The effects of ANXA5 overexpression on increasing the sensitivity of HCT116 cells to the cytotoxic effects of cisplatin on viability, apoptosis, and cell cycle were assessed using MTT assay, annexin V/7ADD flow cytometry, and cell cycle assay. The effects of the treatments on the expression of Bax and Bcl2 were measured using q-PCR.
Results: The overexpression of ANXA5 was confirmed by q-PCR (p<0.001). Cisplatin induced cell death, apoptosis, and cell cycle arrest. The effects of cisplatin on cell death (p = 0.026), apoptosis (p=0.001), and the Bax/Bcl2 ratio(p=0.032) were enhanced by ANXA5 overexpression. However, ANXA5 had no significant effect on the cell cycle arrest induced by cisplatin.
Conclusion: These findings suggest that ANXA5 can sensitize HCT116 cells to the cytotoxic effects of cisplatin.

Keywords

Annexin A5
Chemotherapy
Colorectal cancer
Apoptosis
Cell cycle
Subjects
Journal of Kerman University of Medical Sciences

Articles in Press, Accepted Manuscript
Available Online from 12 April 2026

Article View 1,200

Home

Editorial Board

Guide for Authors

Submit Manuscript

Guide for Reviewers

Contact Us