Document Type : Original Article
Authors
- Arian Amirkhosravi 1
- Maryamossadat Mirtajaddini Goki 2
- Payam Torkzadeh 3
- Maryam Iranpour 4
- Mehrnaz Mehrabani 2
- Ali Mandegary 5
- Shiva Amiri 6
1 Herbal and Traditional Medicines Research Center, Institute of Pharmaceutical Sciences, Kerman University of Medical Sciences, Kerman, Iran.
2 Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
3 Student Research Committee, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
4 Department of Pathology, Pathology and Stem Cell research Center, Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
5 Department of Toxicology and Pharmacology, Faculty of Pharmacy, Shahid Sadoughi, University of Medical Sciences, Yazd, Iran.
6 Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran and Department of Toxicology and Pharmacology, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
Abstract
Background: Lung fibrosis is a chronic disease that affects the lung interstitium and is the primary side effect of bleomycin. Reactive oxygen species (ROS) and inflammatory cells are among the factors that contribute to the fibrotic process. Melatonin is reported to exhibit antioxidant and anti-inflammatory activities. Therefore, the current research aimed to evaluate the impact of melatonin on bleomycin-induced lung fibrosis.
Methods: In total, 30 Wistar rats were randomly assigned to five groups: saline (1 mL/kg), bleomycin (5 mg/kg, i.t.), or three groups receiving bleomycin (5 mg/kg, i.t.) and melatonin (5, 10, and 20 mg/kg/day, i.p.). After the treatment period, the level of hydroxyproline, transforming growth factor‑β1 (TGF‑β1), malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD) and the pathological changes were assessed in the lung tissues.
Results: The results revealed that administration of bleomycin caused pulmonary fibrosis in rats as evidenced by characteristic structural changes in histopathology, elevated lipid peroxidation marker, levels of TGF-β1 and hydroxyproline and reduced GSH and SOD in bleomycin group. All these changes were ameliorated by melatonin (10 and 20 mg/kg/day).
Conclusion: Melatonin alleviate the bleomycin-induced lung fibrosis due to its antioxidant and anti-inflammatory properties.
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