Document Type : Original Article
Authors
- Fatemeh Azadegan-Dehkordi 1
- Maryam Ghasemi-Dehnoo 2
- Masih Hosseini 3
- Sepehr Ebrahimi-Dehkordi 4
- Mehri Qajari 5
- Hossein Amini-Khoei 5
- Zahra Lorigooini 5
- Nader Bagheri 6
1 Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
2 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
3 Cancer Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
4 Student Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran
5 Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
6 Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
Abstract
Introduction: Ulcerative colitis (UC) is a chronic inflammatory disease caused by an imbalance in the immune system. However, the exact role of IL-10 family cytokines in the development of this disease remains unclear. This study was designed to assess the effects of quinic acid (QA), coumaric acid (CA), ferulic acid (FA), and syringic acid (SA) on IL-10 family cytokines in an experimental model of colitis.
Methods: A total of 128 male Wistar rats were divided into 16 groups (n=8 per group). The groups included control, UC, dexamethasone (2 mg/kg/day), and treatment groups receiving different doses (mg/kg/day) of CA (150, 100, 50), SA (50, 25, 10), FA (60, 40, 20), or QA (100, 60, 30, 10). All phenolic acids were given orally by gavage for 5 days. Expression of IL-10 family cytokines (IL-26, IL-24, IL-20, IL-19, and IL-10) was measured by real-time PCR.
Results: Acetic acid administration significantly decreased IL-10 and IL-19 and increased IL-20, IL-24, and IL-26 mRNA expression in the colon tissue. IL-10 and IL-19 expression was significantly upregulated by dexamethasone, QA (100), CA (150), SA (50), and FA (60) compared with the UC group. IL-20 and IL-24 expression were significantly decreased by dexamethasone, QA (60, 100), CA (100, 150), SA (25, 50), and FA (40, 60). IL-26 expression was also significantly reduced by QA (60, 100), CA (150), SA (50), and FA (60).
Conclusion: High doses of QA, CA, SA, and FA increased IL-10 and IL-19 expression and decreased IL-20, IL-24, and IL-26 expression in rats with UC.
Highlights
Fatemeh Azadegan-Dehkordi (Google Scholar)
Maryam Ghasemi-Dehnoo (Google Scholar)
Masih Hosseini (Google Scholar)
Sepehr Ebrahimi-Dehkordi (Google Scholar)
Mehri Qajari (Google Scholar)
Hossein Amini-Khoei (Google Scholar)
Zahra Lorigooini (Google Scholar)
Nader Bagheri* (Google Scholar)
Keywords
Main Subjects
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