Journal of Kerman University of Medical Sciences

The Effects of Morning Versus Evening Aerobic Exercise Training on Proteins Associated with Browning of Visceral Adipose Tissue in a Diabetic Mouse Model

Document Type : Original Article

Authors

Maryam Janbozorgi
Mehrzad Shabani
Masoumeh Hosseinzadeh
Zahra Hemati Farsani

Department of Sports Physiology, Faculty of Sports Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

https://doi.org/10.34172/jkmu.4119
Abstract
Introduction: Converting white adipose tissue to brown-like fat (browning) has shown promise in reducing diabetes-associated complications. This study aimed to determine the effect of aerobic exercise training time on CPT1, CLOCK, and irisin protein levels in diabetic mice.
Methods: In this experimental study, 18 male NMRI mice (8-10 weeks old, mean weight 26 ± 2.23 g) were randomized into six groups (five animals/group): Healthy controls (light and dark phases), diabetic controls (light and dark phases), and diabetic exercise intervention groups (light and dark phases). Moderate-intensity aerobic exercise (50-60% of maximum running speed) was performed for eight weeks. Differences among experimental groups were statistically analyzed using two-way ANOVA, followed by Tukey’s HSD test.
Results: Significantly elevated levels of CPT1, CLOCK, and irisin were observed in the healthy control group compared to the diabetic control group (P = 0.0001). In the dark-phase diabetic exercise training group, CPT1 (P = 0.001), CLOCK (P = 0.001), and irisin (P = 0.001) protein levels were significantly elevated compared to the diabetic control group. However, morning exercise training did not show a significant difference compared to the diabetic control group (P > 0.05).
Conclusion: In this animal model, our results indicate that aerobic exercise undertaken during the dark phase could positively influence adipose tissue metabolism in diabetic conditions.

Highlights

 Zahra Hemati Farsani (Google Scholar) (PubMed)

 Maryam Janbozorgi (Google Scholar) (PubMed)

Keywords

Diabetes mellitus
Aerobic exercise
Circadian rhythm
Adipose tissue
Brown
FNDC5 protein
Carnitine O-Palmitoyl transferase
Clock proteins
Subjects
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Journal of Kerman University of Medical Sciences
Volume 33
Pages 1-8

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