Journal of Kerman University of Medical Sciences

Current Issue

By Issue

By Author

Author Index

Keyword Index

About Journal

Aims and Scope

JKMU Ethical Policies

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

News

Metric

The Effects of Morning Versus Evening Aerobic Exercise Training on Proteins Associated with Browning of Visceral Adipose Tissue in a Diabetic Mouse Model

Document Type : Original Article

Authors

Department of Sports Physiology, Faculty of Sports Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Introduction: Converting white adipose tissue to brown-like fat (browning) has shown promise in reducing diabetes-associated complications. This study aimed to determine the effect of aerobic exercise training time on CPT1, CLOCK, and irisin protein levels in diabetic mice.
Methods: In this experimental study, 18 male NMRI mice (8-10 weeks old, mean weight 26 ± 2.23 g) were randomized into six groups (five animals/group): Healthy controls (light and dark phases), diabetic controls (light and dark phases), and diabetic exercise intervention groups (light and dark phases). Moderate-intensity aerobic exercise (50-60% of maximum running speed) was performed for eight weeks. Differences among experimental groups were statistically analyzed using two-way ANOVA, followed by Tukey’s HSD test.
Results: Significantly elevated levels of CPT1, CLOCK, and irisin were observed in the healthy control group compared to the diabetic control group (P = 0.0001). In the dark-phase diabetic exercise training group, CPT1 (P = 0.001), CLOCK (P = 0.001), and irisin (P = 0.001) protein levels were significantly elevated compared to the diabetic control group. However, morning exercise training did not show a significant difference compared to the diabetic control group (P > 0.05).
Conclusion: In this animal model, our results indicate that aerobic exercise undertaken during the dark phase could positively influence adipose tissue metabolism in diabetic conditions.

Highlights

 Zahra Hemati Farsani (Google Scholar) (PubMed)

 Maryam Janbozorgi (Google Scholar) (PubMed)

Keywords

Main Subjects

References
  1. Jin Y, Wan K, Liu C, Cheng W, Wang R. Mechanisms of exercise intervention in type 2 diabetes: a bibliometric and visualization analysis based on CiteSpace. Front Endocrinol (Lausanne) 2024;15:1401342. doi:10.3389/fendo.2024.1401342
  2. Šiklová M, Šrámková V, Koc M, Krauzová E, Čížková T, Ondrůjová B, et al. The role of adipogenic capacity and dysfunctional subcutaneous adipose tissue in the inheritance of type 2 diabetes mellitus: cross-sectional study. Obesity (Silver Spring) 2024;32(3):547-59. doi:10.1002/oby.23969
  3. Ahangarpour A, Oroojan AA, Khorsandi L, Kouchak M, Badavi M. Hypolipidemic and hepatoprotective effects of myricitrin and solid lipid nanoparticle-containing myricitrin on the male mouse model with type 2 diabetes induced by streptozotocin-nicotinamide. J Kerman Univ Med Sci 2021;28(1):32-42. doi:10.22062/jkmu.2021.91562
  4. Zhou H, Li T, Li J, Zhuang X, Yang J. The association between visceral adiposity index and risk of type 2 diabetes mellitus. Sci Rep 2024;14(1):16634. doi:10.1038/s41598-024-67430-x
  5. Man AW, Xia N, Li H. Circadian rhythm in adipose tissue: novel antioxidant target for metabolic and cardiovascular diseases. Antioxidants (Basel) 2020;9(10):968. doi:10.3390/ antiox9100968
  6. Li H, Zhang Y, Wang F, Donelan W, Zona MC, Li S, et al. Effects of irisin on the differentiation and browning of human visceral white adipocytes. Am J Transl Res 2019;11(12):7410-21.
  7. Motta VF, Bargut TL, Aguila MB, Mandarim-de-Lacerda CA. Treating fructose-induced metabolic changes in mice with high-intensity interval training: insights in the liver, white adipose tissue, and skeletal muscle. J Appl Physiol (1985) 2017;123(4):699-709. doi:10.1152/japplphysiol.00154.2017
  8. Anastasilakis AD, Polyzos SA, Saridakis ZG, Kynigopoulos G, Skouvaklidou EC, Molyvas D, et al. Circulating irisin in healthy, young individuals: day-night rhythm, effects of food intake and exercise, and associations with gender, physical activity, diet, and body composition. J Clin Endocrinol Metab 2014;99(9):3247-55. doi:10.1210/jc.2014-1367
  9. Petrenko V, Sinturel F, Riezman H, Dibner C. Lipid metabolism around the body clocks. Prog Lipid Res 2023;91:101235. doi:10.1016/j.plipres.2023.101235
  10. Abdi A, Mehrabani J, Nordvall M, Wong A, Fallah A, Bagheri R. Effects of concurrent training on irisin and fibronectin type-III domain containing 5 (FNDC5) expression in visceral adipose tissue in type-2 diabetic rats. Arch Physiol Biochem 2022;128(3):651-6. doi:10.1080/13813455.2020.1716018
  11. Haj Hosseini M, Shahouzehi B, Hosseini NS. The effect of high-intensity interval training in the presence of L-carnitine on the expression of MCT1, PGC-1, and CS in the liver of male Wistar rats. J Kerman Univ Med Sci 2024;31(5):238-42. doi:10.34172/jkmu.2024.37
  12. da Cruz Rodrigues KC, Pereira RM, de Campos TD, de Moura RF, da Silva AS, Cintra DE, et al. The Role of Physical Exercise to Improve the Browning of White Adipose Tissue via POMC Neurons. Front Cell Neurosci 2018;12:88. doi:10.3389/ fncel.2018.00088
  13. Ghasemi A, Jeddi S. Streptozotocin as a tool for induction of rat models of diabetes: a practical guide. EXCLI J 2023;22:274-94. doi:10.17179/excli2022-5720
  14. Chavanelle V, Boisseau N, Otero YF, Combaret L, Dardevet D, Montaurier C, et al. Effects of high-intensity interval training and moderate-intensity continuous training on glycaemic control and skeletal muscle mitochondrial function in db/db mice. Sci Rep 2017;7(1):204. doi:10.1038/s41598-017-00276-8
  15. Sato S, Basse AL, Schönke M, Chen S, Samad M, Altıntaş A, et al. Time of exercise specifies the impact on muscle metabolic pathways and systemic energy homeostasis. Cell Metab 2019;30(1):92-110.e4. doi:10.1016/j.cmet.2019.03.013
  16. Dalbram E, Basse AL, Zierath JR, Treebak JT. Voluntary wheel running in the late dark phase ameliorates diet-induced obesity in mice without altering insulin action. J Appl Physiol (1985) 2019;126(4):993-1005. doi:10.1152/japplphysiol.00737.2018
  17. Albrecht U, Eichele G. The mammalian circadian clock. Curr Opin Genet Dev 2003;13(3):271-7. doi:10.1016/s0959- 437x(03)00055-8
  18. Monsalves-Alvarez M, Morales PE, Castro-Sepulveda M, Sepulveda C, Rodriguez JM, Chiong M, et al. β-Hydroxybutyrate increases exercise capacity associated with changes in mitochondrial function in skeletal muscle. Nutrients 2020;12(7)1930. doi:10.3390/nu12071930
  19. Ertuna GN, Sahiner ES, Yilmaz FM, Ates I. The role of irisin and asprosin level in the pathophysiology of prediabetes. Diabetes Res Clin Pract 2023;199:110642. doi:10.1016/j. diabres.2023.110642
  20. Nema Al-Aridhi DT, Allehibi KI, Razak Al-Sharifi ZA, Al Quraishi M. Serum levels of novel biochemical marker (irisin) in relation to the duration of type 2 diabetes & in cases of type 2 diabetes with coronary artery disease in Iraqi patients aged (40- 60 year). Med Leg Update 2020;20(1):606-12. doi:10.37506/ mlu.v20i1.429
  21. Montazerifar F, Karajibani M, Sedaghat G, Shourestani S, Azar Nour F, Bolouri A. The relationship between serum levels of irisin with cardiometabolic biomarkers in patients with type 2 diabetes. J Nutr Food Secur 2022;7(4):445-51. doi:10.18502/ jnfs.v7i4.11055
  22. Tentolouris A, Eleftheriadou I, Tsilingiris D, Anastasiou IA, Kosta OA, Mourouzis I, et al. Plasma irisin levels in subjects with type 1 diabetes: comparison with healthy controls. Horm Metab Res 2018;50(11):803-10. doi:10.1055/a-0748-6170
  23. Moreno-Navarrete JM, Ortega F, Serrano M, Guerra E, Pardo G, Tinahones F, et al. Irisin is expressed and produced by human muscle and adipose tissue in association with obesity and insulin resistance. J Clin Endocrinol Metab 2013;98(4): E769-78. doi:10.1210/jc.2012-2749
  24. Zhang Y, Li R, Meng Y, Li S, Donelan W, Zhao Y, et al. Irisin stimulates browning of white adipocytes through mitogen-activated protein kinase p38 MAP kinase and ERK MAP kinase signaling. Diabetes 2014;63(2):514-25. doi:10.2337/db13-1106
  25. Muscella A, Stefàno E, Lunetti P, Capobianco L, Marsigliante S. The regulation of fat metabolism during aerobic exercise. Biomolecules 2020;10(12):1699. doi:10.3390/biom10121699
  26. Qu Q, Zeng F, Liu X, Wang QJ, Deng F. Fatty acid oxidation and carnitine palmitoyltransferase I: emerging therapeutic targets in cancer. Cell Death Dis 2016;7(5): e2226. doi:10.1038/ cddis.2016.132
  27. Kim HJ, Kim YJ, Seong JK. AMP-activated protein kinase activation in skeletal muscle modulates exercise-induced uncoupled protein 1 expression in brown adipocyte in mouse model. J Physiol 2022;600(10):2359-76. doi:10.1113/jp282999
  28. Cho YK, Lee S, Lee J, Doh J, Park JH, Jung YS, et al. Lipid remodeling of adipose tissue in metabolic health and disease. Exp Mol Med 2023;55(9):1955-73. doi:10.1038/s12276-023- 01071-4
  29. Chou TJ, Lu CW, Lin LY, Hsu YJ, Huang CC, Huang KC. Proteomic analysis of skeletal muscle and white adipose tissue after aerobic exercise training in high fat diet induced obese mice. Int J Mol Sci 2023;24(6):5743. doi:10.3390/ ijms24065743
  30. Shen B, Ma C, Wu G, Liu H, Chen L, Yang G. Effects of exercise on circadian rhythms in humans. Front Pharmacol 2023;14:1282357. doi:10.3389/fphar.2023.1282357
  31. Zhang Z, Li X, Zhang J, Du J, Zhang Q, Ge Z, et al. Chrono-aerobic exercise optimizes metabolic state in DB/DB mice through CLOCK-mitophagy-apoptosis. Int J Mol Sci 2022;23(16):9308. doi:10.3390/ijms23169308
  32. Bennett S, Sato S. Enhancing the metabolic benefits of exercise: is timing the key? Front Endocrinol (Lausanne) 2023;14:987208. doi:10.3389/fendo.2023.987208
  33. Liu C, Li S, Liu T, Borjigin J, Lin JD. Transcriptional coactivator PGC-1alpha integrates the mammalian clock and energy metabolism. Nature 2007;447(7143):477-81. doi:10.1038/ nature05767
  34. Yu R, Tian L, Ding Y, Gao Y, Li D, Tang Y. Correlation between inflammatory markers and impaired circadian clock gene expression in type 2 diabetes mellitus. Diabetes Res Clin Pract 2019;156:107831. doi:10.1016/j.diabres.2019.107831
  35. Ayari S, Abellard A, Carayol M, Guedj É, Gavarry O. A systematic review of exercise modalities that reduce pro-inflammatory cytokines in humans and animals’ models with mild cognitive impairment or dementia. Exp Gerontol 2023;175:112141. doi:10.1016/j.exger.2023.112141
  36. Wolff CA, Esser KA. Exercise sets the muscle clock with a calcium assist. J Physiol 2020;598(24):5591-2. doi:10.1113/ jp280783
  37. Martin RA, Esser KA. Time for exercise? Exercise and its influence on the skeletal muscle clock. J Biol Rhythms 2022;37(6):579-92. doi:10.1177/07487304221122662
  38. Hayes LD, Bickerstaff GF, Baker JS. Interactions of cortisol, testosterone, and resistance training: influence of circadian rhythms. Chronobiol Int 2010;27(4):675-705. doi:10.3109/07420521003778773

 

Journal of Kerman University of Medical Sciences
Volume 33
Pages 1-8
Files
Share
How to cite
Statistics