Journal of Kerman University of Medical Sciences

The Effect of Inflammation on Pentylenetetrazol Induced Seizures in Rats

Document Type : Original Article

Authors

Maryam Rameshk 1
Mohammad Khaksari Hadad 2
Hossein Ali Ebrahimi Meimand 1
Alireza Raji-Amirhasani 3

1 Neurology Research Center, Kerman University of Medical Sciences, Kerman, Iran

2 Endocrinology and Metabolism Research Center, Kerman University of Medical Sciences, Kerman, Iran

3 Sirjan School of Medical Sciences, Sirjan, Iran

https://doi.org/10.34172/jkmu.4164
Abstract
Introduction: Epilepsy is a common and debilitating neurological disorder associated with reduced quality of life and increased mortality. This study investigated the hypothesis of Traditional Persian Medicine about the effect of local inflammation on seizures using a rat model.
Methods: Adult male Wistar rats (n = 28) were divided into four groups: One control group (CTL) and three experimental groups in which epilepsy was induced 6 (INF-EPI-6), 14 (INF-EPI-14), or 28 days (INF-EPI-28) after carrageenan-induced paw inflammation. Seizures were triggered using intraperitoneal injection of pentylenetetrazol (PTZ). Seizure latency, duration, severity, mortality, and serum levels of IL-6 and CRP were assessed.
Results: Serum IL-6 levels significantly increased in the experimental groups compared to control (CTL: 7.16 ± 1.88 pg/ml; INF-EPI-6: 24.10 ± 1.61, P < 0.01; INF-EPI-14: 13.32 ± 1.03, P < 0.05; INF-EPI-28: 14.01 ± 1.15, P < 0.05). CRP levels remained unchanged across groups. Seizure severity significantly decreased in INF-EPI-14 (1.14 ± 0.45) vs. control (3 ± 0.37, P < 0.05), though latency and duration showed no significant differences. IL-6 levels were negatively correlated with seizure severity in INF-EPI-6 (P < 0.05), and CRP levels showed a similar correlation in INF-EPI-14 (P < 0.01).
Conclusion: Pre-existing peripheral inflammation may reduce seizure severity, partially supporting Jorjani’s theory. However, further studies are needed to elucidate the underlying mechanisms.

Highlights

 Maryam Rameshk  (Google Scholar) (PubMed)

 Hossein Ali Ebrahimi Meimand  (Google Scholar) (PubMed)

Keywords

Carrageenan
Epilepsy
Seizures
Persian medicine
Pentylenetetrazol
Inflammation
Subjects
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Journal of Kerman University of Medical Sciences
Volume 33
Pages 1-8

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