Document Type : Original Article
Authors
- Mahla Shahrokhi Sardoo 1
- Ali Derakhshani 2
- Seyedeh Mahdieh Khoshnazar 3
- Nader Shahrokhi 4
- Ayat Kaeidi 1
1 Physiology-Pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
2 Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran
3 Gastroenterology and Hepatology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran
4 Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
Abstract
Introduction: Chronic and acute cutaneous wounds remain a major clinical burden due to impaired healing dynamics, persistent inflammation, and the risk of infection or scarring. The search for novel, biocompatible, and non-invasive therapeutic strategies has therefore intensified in recent years.
Methods: In this study, we investigated the regenerative potential of exosomes derived from human dermal fibroblasts and exosomes derived from ginger extract in promoting the healing of open wounds healing in rats. Twenty-eight male Wistar rats were randomly divided into four groups: negative control (no wound), positive control (saline-treated wound), fibroblast-derived exosome-treated, and ginger-derived exosome -treated. A 12-mm full-thickness dorsal wound was created in each rat, and topical treatments were applied once daily for 15 days. Exosomes from both sources were isolated via ultracentrifugation and characterized using transmission electron microscopy and protein quantification assays.
Results: Macroscopic and histological analyses demonstrated that both fibroblast-derived and ginger-derived exosome treatments significantly accelerated wound closure compared with the positive control group (p < 0.001). Throughout the 15-day observation period, the wound area in both treatment groups decreased progressively and, by day 15, was nearly completely healed, showing no significant difference from the negative control group (non-wounded animals). Treated wounds exhibited enhanced epithelial regeneration, increased collagen deposition, and reduced inflammatory cell infiltration relative to the positive control group.
Conclusion: These findings demonstrate that dermal fibroblast-derived and ginger-derived exosomes enhance tissue repair and accelerate wound healing in rats. Overall, the results highlight their potential as safe, natural, and effective bio-nano agents for promoting cutaneous wound regeneration.
Highlights
Ali Derakhshani (Google Scholar) (PubMed)
Seyedeh Mahdieh Khoshnazar (Google Scholar) (PubMed)
Nader Shahrokhi (Google Scholar) (PubMed)
Ayat Kaeidi (Google Scholar) (PubMed)
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