Background & Aims: Based on serological studies the prevalence of Helicobacter Pylori infection in Iranian adults is up to 80%. Gastritis, peptic ulcer and gastric adenocarcinoma are common clinical outcomes of this infection in Iran. Since antibiotic resistance patterns of Helicobacter pylori are different geographically, local studies are highly required. Method: From April to December 2009, 63 isolates of Helicobacter pylori were obtained From 191 patients referred to the endoscopy unit of Afzalipour hospital in Kerman. Demographic features including age, gender, symptoms, … were recorded before the sampling and sensitivity to six common antibiotics used for the treatment of H.pylori infection was determined. Modified disk diffusion test was used to evaluate antibiotic resistance pattern. Data analysis was done through SPSS 16 and using Pearson chi-square test. Results: The patterns of antibiotics resistance were as below: metronidazole 55.5%, clarithromycin 30.1%, tetracycline 3.1%, amoxicillin 26.9%, ciprofloxacin 7.9% and no resistance to furazolidone was detected. While 12.7% of the isolates were susceptible to all the six antibiotics, 55.6% were resistant to one antibiotic, 25.4% to two antibiotics, 6.3% to three antibiotics and there was no resistance to more than three antibiotics at the same time. Conclusion: According to the obtained antibiotic resistance rates in this study, performing antibiogram tests before starting the treatment is necessary.