Document Type : Original Article
Neurology Research Center, Kerman University of Medical Science, Kerman, Iran
Department of Genetics, Kerman University of Medical Science, Kerman, Iran
Department of Immunology, Medical School, Kerman University of Medical Sciences, Kerman, Iran
Department of Immunology, Medical School, Kerman University of Medical Sciences, Kerman, Iran & Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Background: Recent evidences revealed that some genetic factors strongly predict occurrence of lamotrigine (LTG)-related skin reactions. The present study aimed to assess the association between some human leukocyte antigen (HLA)-B alleles and risk of LTG-related skin reactions among a sample of epileptic patients.
Methods: Totally, 36 epileptic patients expressing LTG-related skin reactions and 70 sex- and age-matched healthy individuals were enrolled into this case-control study. Blood samples were collected from all participants and genomic DNA was extracted by salting-out method. HLA-B alleles were determined using standard sequence specific primer-PCR (SSP-PCR) technique.
Results: Of the 31 HLA alleles assessed in our survey, the frequencies of HLA-B*38 and HLA-B*40 were significantly higher in epileptic patients with LTG-related skin reactions when compared to the control group. In term of gender, the frequency of HLA-B*40 allele was significantly higher in the epileptic men with LTG-related skin reactions, whereas the frequency of HLA-B*38 allele was significantly higher in the epileptic women with LTG-related skin reactions than controls with the same gender. Moreover, the frequency of HLA-B*38 allele in patients with high grade of LTG-related skin side effects was significantly higher than patients with low grade of LTG-related skin side effects.
Conclusion: These results indicated possible association between HLA-B*40 and HLA-B*38 alleles and LTG-induced skin lesions in Iranian epileptic patients. HLA-B*40 and HLA-B*38 alleles might be differentially expressed in male and female epileptic patients with LTG-induced skin lesions.